Power up your sequencing applications with circular DNA
Nicking Loop™ is redefining DNA as a dynamic, interactive medium for data storage and access. By enabling information to be stored and retrieved directly on DNA—capabilities once exclusive to digital databases—we are unlocking revolutionary possibilities for data-driven healthcare and scientific research.
PCR-free DNA amplification
High-fidelity queries and copies
Platform-agnostic sequencing
Designed for simplicity, evenness and uniformity
Applications
Whole Genome Sequencing
Platform-agnostic read-out for circular libraries, optimized for comprehensive genome coverage with exceptional evenness and uniformity, ensuring high accuracy across all genomic regions.
Whole Exome Sequencing
Designed for circular libraries, Nicking Loop™ ensures uniform and even read-out, maximizing coverage of exonic regions and delivering precise results for targeted analysis.
Methylation Sequencing
Platform-agnostic read-out tailored for circular libraries, providing consistent, uniform data ideal for sensitive detection of methylation patterns across the genome
Fragmentomics
Engineered for circular libraries, Nicking Loop™ delivers uniform and even read-out, ideal for analyzing fragmented DNA with precision and reliability.
FAQ
Yes.
The Nicking Loop™ workflow has been demonstrated with Opentrons OT-2.
The length of the libraries varies depending on the probe design. The length is usually approximately 225 bases.
Yes.
Bridge Capture™ workflow permits sample pooling after the initial targeting step without the need for normalization whereas other commercially available technologies usually pool the pre-indexed amplified libraries just before sequencing. This approach ensures that as the number of samples processed in parallel increases, the associated increase in workflow duration, cost, and complexity does not follow a linear trend, offering a distinct advantage in efficiency and scalability. Our pooling approach is currently protected within our patents and undergoing further improvements.
In the Nicking Loop™ workflow the samples can be pooled after introducing and ligating the Nicking Loops.