Nicking Loop™ technology

Circular sequencing by Geno1® Technologies

More about Nicking Loop™ applications
How does it work?

Nicking Loop™

Nicking Loop™ copying – PCR-free DNA amplification

Nicking Loop™ copying is a PCR-free amplification method for circular DNA, suited for high-fidelity information read-out and database-like operations. 1. A loop-specific oligonucleotide is annealed with the Nicking Loop™-converted DNA. 2. Rolling circle amplification is primed to create concatemeric copies of the circular DNA. 3. The looped part of the concatemer is cleaved, resulting in monomerization of the concatemer. 4. The monomers are self-circularized to result in amplified circular DNA.

Nicking Loop™ DNA conversion & indexing

In Nicking Loop™ conversion, a looped index in ligated to the DNA molecules to be converted. 1. Y-adapters are ligated to end-repaired DNA. 2. The looped index is brought into contact with the Y-adapted-ligated DNA using a supporting oligo. 3. The supporting oligo is allowed to anneal to the Y-adapter ends. 4. The looped index is ligated to the Y-adapter ends. 5. The supporting oligo is removed, resulting in Nicking Loop™-converted DNA molecules.
NICKING LOOP™

How does it perform

Yes.

The Nicking Loop™ workflow has been demonstrated with Opentrons OT-2.

The length of the libraries varies depending on the probe design. The length is usually approximately 225 bases.

Yes.

Bridge Capture™ workflow permits sample pooling after the initial targeting step without the need for normalization whereas other commercially available technologies usually pool the pre-indexed amplified libraries just before sequencing. This approach ensures that as the number of samples processed in parallel increases, the associated increase in workflow duration, cost, and complexity does not follow a linear trend, offering a distinct advantage in efficiency and scalability. Our pooling approach is currently protected within our patents and undergoing further improvements.

In the Nicking Loop™ workflow the samples can be pooled after introducing and ligating the Nicking Loops.

Patents

Accurate and massively parallel quantification of nucleic acid

  • ZL201880055271.3
  • EP3673081
  • HK40017021
  • JP7074978

Methods for accurate parallel quantification of nucleic acids in dilute or non-purified samples

  • EP4060049
  • US11898202

Highly sensitive methods for accurate parallel quantification of nucleic acids

  • EP4060050
  • US11486003

Methods for accurate parallel detection and quantification of nucleic acids

  • US11970736