Genomill Introduces Nicking Loop™: Pioneering Circular DNA Libraries for Next-Generation Sequencing

Turku, Finland – 12.5.2025 – Genomill is excited to announce the publication of its latest preprint detailing the Nicking Loop™ technology—a groundbreaking approach that positions circular single-stranded DNA (ssDNA) as the primary library format for next-generation sequencing (NGS). This advancement marks a significant departure from traditional linear DNA libraries, offering a simplified and versatile alternative.

Nicking Loop™ method enables non-exponential amplification of circular ssDNA, preserving the original template composition and enhancing reproducibility and sensitivity, particularly for detecting low-frequency variants. This approach is compatible with various NGS platforms, including nanopore sequencing technologies, enabling highly efficient targeted sequencing.

As part of its innovation, Nicking Loop™ introduces an indexing strategy that simplifies sample pooling early in the workflow. This addresses a well-known bottleneck in sample processing, improving scalability while minimizing the risk of cross-contamination, which is an important consideration in clinical and high-throughput settings.

“Nicking Loop™ sets a new standard for NGS library design by shifting the paradigm from linear to circular DNA templates,” said Juha-Pekka Pursiheimo, CTO and inventor of the technology. “This shift enables more flexible, efficient, and robust sequencing workflows.”

Manu Tamminen, CEO and Co-Founder of Genomill, added, “This publication underscores our commitment to innovation in genomic technologies. Nicking Loop™ not only streamlines sequencing workflows but also paves the way for new types of DNA information storage applications.”

For a comprehensive understanding of the Nicking Loop™ technology and its potential applications, we invite you to read the full preprint here: https://doi.org/10.1101/2025.05.08.652165.

For more information on Nicking Loop™, visit https://genomill.com/nicking-loop/.