Nicking loop™ / Technology
Circular sequencing by Geno1® technologies

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PCR-free DNA amplification
Nicking Loop™ enables direct linear amplification of circular ssDNA libraries, eliminating biases and pitfalls associated with PCR amplification.
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High-fidelity queries and copies
Nicking Loop™ transforms DNA libraries into structured databases, enabling efficient operations like data copying and advanced querying.
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Platform-agnostic sequencing
Circular Nicking Loop™ libraries are compatible with any sequencing platform. Libraries can be sequenced directly in their circular form or converted into linear libraries to fit seamlessly with any workflow.
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Designed for simplicity, evenness and uniformity
Nicking Loop™ provides a simple and robust process that minimizes errors while delivering highly even and uniform data for exceptional accuracy and consistency.
Nicking Loop™ copying – PCR-free DNA amplification

Nicking Loop™ copying is a PCR-free amplification method for circular DNA, suited for high-fidelity information read-out and database-like operations.
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01
A loop-specific oligonucleotide is annealed with the Nicking Loop™-converted DNA.
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02
Rolling circle amplification is primed to create concatemeric copies of the circular DNA.
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03
The looped part of the concatemer is cleaved, resulting in monomerization of the concatemer.
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04
The monomers are self-circularized to result in amplified circular DNA.
Nicking Loop™ DNA conversion & indexing

In Nicking Loop conversion, a looped index oligo complex is ligated to DNA molecules, enabling their circularization
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01
Y-adapters are ligated to end-repaired DNA.
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02
The looped index is brought into contact with the Y-adapted-ligated DNA using a supporting oligo.
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03
The supporting oligo is allowed to anneal to the Y-adapter ends.
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04
The looped index is ligated to the Y-adapter ends.
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05
The supporting oligo is removed, resulting in Nicking Loop™-converted DNA molecules.
NICKING LOOP™
How does it perform?

