Bridge Capture / Technology

Targeted sequencing by Geno1® technologies

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  • Affordable testing

    Bridge Capture™ is engineered for cost-efficiency, utilizing off-the-shelf reagents and optimizing the use of sequencing capacity to minimize test costs.

  • Sensitive detection

    Bridge Capture™ delivers proven sensitivity, meeting the rigorous demands of even the most challenging oncological applications.

  • Portable workflows

    Bridge Capture™ is designed for simplicity, portability, and kittability, ensuring seamless adoption in any laboratory setting, effortless usability in distributed environments, and compatibility with any sequencing platform.

  • Pan-cancer detection

    Bridge Capture™ supports panels of any size, enabling true pan-cancer detection across any number and type of mutations.

Image describing technical bridge capture

Bridge Capture™ is a laboratory process designed for simple, sensitive and cost-efficient targeting of DNA sequencing.

  • 01

    At the beginning of the process, the probe-bridge complexes are circularized around their genomic targets.

  • 02

    Linear DNA is degraded, leaving only circularized probes.

  • 03

    Circular probes are amplified using rolling circle amplification.

  • 04

    The concatemers are indexed using limited-cycle PCR.

  • 05

    The circular probes can be directly sequenced on NGS platforms using circular sequencing templates.

  • 06

    The concatemers can be sequenced on nanopore platforms.

  • 07

    The PCR-indexed templates can be sequenced on Illumina or Ion Torrent platforms.

How does it perform?



Bridge capture™

Patents

Methods for accurate parallel detection and quantification of nucleic acids
Highly sensitive methods for accurate parallel quantification of nucleic acids
Methods for accurate parallel quantification of nucleic acids in dilute or non-purified samples
Accurate and massively parallel quantification of nucleic acid