Power up your sequencing applications with circular DNA

Yes. The Bridge Capture™ and Nicking Loop™ have been designed to produce sequencing libraries that are compatible with any NGS platform. This is made possible by the circular libraries (see question How is the Nicking Loop™ conversion & indexing done?) which can be sequenced either with short-read or long-read platforms. See question What are the outputs of Bridge Capture™ and Nicking Loop™ processes? for an overview of how the Bridge Capture™ and Nicking Loop™ technology outputs fit to different sequencing platforms.

Yes. The Nicking Loop™ conversion & indexing (see question How is the Nicking Loop™ conversion & indexing done?) enables the creation of circular single-stranded DNA (ssDNA) whole-genome libraries, facilitating comprehensive whole-genome sequencing.

Yes. The smallest amount of patient cfDNA tested with Bridge Capture™ thus far has been approximately 2000 genomic copies (5 ng). The detection of low allelic fractions benefits from a larger amount of sample used in the workflow. With Nicking Loop™ we have not conducted large enough studies on this topic yet.

Yes. Bridge Capture™ can analyze variety of the different sample materials. Routinely tested sample types are cfDNA, gDNA, and cDNA. The technology has demonstrated promising results on direct targeting of RNA, as well as capturing unpurified DNA from urine and saliva. With Nicking Loop™ we are currently validating the dynamic range of the method.

Yes. Bridge Capture™ workflow permits sample pooling after the GapFill (see question How does GapFill work in the Bridge Capture™?) without the need for normalization whereas other commercially available technologies usually pool the pre-indexed amplified libraries just before sequencing. This approach ensures that as the number of samples processed in parallel increases, the associated increase in workflow duration, cost, and complexity does not follow a linear trend, offering a distinct advantage in efficiency and scalability. Our pooling approach is currently protected within our patents and it is undergoing further improvements. When using Nicking Loops, the samples can be pooled after introducing and ligating the Nicking Loops. This would be after GapFill when using Nicking Loops in Bridge Capture™ (see question How does GapFill work in the Bridge Capture™?). In case of Nicking Loop™ conversion and indexing the pooling can be done after the circularization step (see question How is the Nicking Loop™ conversion & indexing done?).

No. Both the Bridge Capture™ and the Nicking Loop™ workflows can be completed using standard molecular laboratory equipment such as pipettes, vortexes, spinners, and PCR instrument. No specialized or uncommon equipment is necessary to successfully carry out the protocol.