Bridge Capture™ process is illustrated in Figure 1 and the technology is illustrated in detail in Figure 2.
Bridge Capture™ is an efficient targeted pre-sequencing technology which offers a rapid, highly sensitive, platform agnostic and cost-effective molecular quantification solution.
In Bridge Capture™ the sample is targeted with Bridge Capture™ probes (see question
How does the targeting work in the Bridge Capture™?) are included in the probes.
Once the probes have found their intended targets, the gap between probes is copied from the target sequence and ligated to form gap-filled circular probe construct (see question
How does GapFill work in the Bridge Capture™?), a circular library, which can be optionally sequenced directly with a circular-based sequencer like Complete Genomic's DNBSEQ, Element's Aviti or PacBio's Onso. This circular library is linearly amplified by RCA to generate multiple copies of the circular probe (see questions
How does the rolling circle amplification (RCA) work in Bridge Capture™ and Nicking Loop™?), which can be optionally sequenced with long-read sequencing platforms, such as Oxford Nanopore Technologies.
After linear amplification, sequencing platform-specific adapters are introduced through a limited-cycle indexing PCR (see question
How does the limited-cycle indexing PCR work in the Bridge Capture™?). To ensure optimal quality and purity, the resulting libraries are purified with bead purification protocols. This step helps to remove any impurities and contaminants that may affect the accuracy of the sequencing data.
[caption id="attachment_894" align="alignnone" width="1530"]
Figure 1 Complete Bridge Capture™ workflow comprises probe mix, process and reagents, and Geno1® data processing.[/caption]
[caption id="attachment_893" align="alignnone" width="1644"]
Figure 2 Bridge Capture™ workflow depicted in step-by-step manner. Probes hybridize to their targets and the gap is filled and the circular structure is formed by ligation. RCA is primed from the bridge and the adapter sequences are introduced in a limited-cycle indexing PCR.[/caption]